Meteorin role in neuronal development
Dates:2 January 2018 - 30 March 2018
Date limite de candidature:30 March 2018
~ Jan-March 2018
Meteorin and Meteorin-like are newly discovered secreted proteins involved in both glia and neuronal cell differentiation. More recently, they have also been implicated in axonal growth in in vitro systems. We have cloned the zebrafish orthologous gene (Meteorin, Metrnl1 and Metrnl2) and determined their expression during CNS development in larval zebrafish. We have created null alleles of all three genes via CRISPR/Cas9 technology. Although the mutant fish are viable they show very penetrant and specific axonal growth defect. Preliminary work has demonstrated that these axons fail to innervate the proper brain areas and establish the correct synaptic connectivity. The student will characterize in detail these defects in trigeminal ganglion neuron axons using transgenic lines, in vivo imaging and immunohistochemical analysis. He/she will as well analyze double and triple mutants for these genes that have already been generated, to unmask new phenotypes, including abnormalities in commissural axon crossing and visual perception defects via calcium imaging of behaving animals.
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