Cortical dynamics and coding mechanisms

Leader

Research center

45 rue d’Ulm
75230 Paris
Marc Mézard

Institution

Ecole Normale Supérieure
Inserm
CNRS
ED158
Université Pierre et Marie Curie

Laboratory

Institut de Biologie de l'ENS IBENS
U1024 UMR8197
Labex Memolife, Programme France Bio-Imaging

Mots clefs

Integrative neuroscience
Tactile sensory modality
Barrel cortex
Two-photon fluorescence microscopy
Adaptive optics
Available to host a PhD student

publications

Wang J., Leger J.-F., Binding J., Boccara C., Gigan S., Bourdieu L., Measuring aberrations in the rat brain by coherence-gated wavefront sensing using a Linnik(2012)

J. Ben Arous, J. Binding, J.-F. Léger, M. Casado, S. Gigan, C. Boccara, L. Bourdieu. Single myelin fiber imaging in living rodents without labeling by deep optical coherence microscopy J. Biomed. Opt. 16(11) 116012. (2011)

Y. Kremer, J.-F. Léger, D. Goodman, R. Brette, L. Bourdieu. Late maturation of the vibrissae direction selectivity map in the rat barrel cortex. J. Neurosci. 31(29) 10689 –10700. (2011)

J. Binding, J. Ben Arous, J.-F. Léger, S. Gigan, C. Boccara and L. Bourdieu. Brain refractive index measured in vivo with high-NA defocus-corrected full-field OCT and its consequence on two-photon microscopy. Opt. Express, 19(6) 4833-47.  (2011)

Y. Kremer, J.-F. Léger, R. Lapole, N. Honnorat, Y. Candela, S. Dieudonné and L. Bourdieu A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view. Opt. Express 16(14), 10066-10076.(2008) 

Fields of research

Neurophysiology / systems neuroscience

Research Theme

We study the representation of sensory information in a cortical column by recording optically the activity of tens to thousands of neurons. We are interested in particular in the tactile information collected by the rodent whiskers and encoded in the barrel field of the primary somato-sensory cortex. Our work focuses on the coding of elementary stimuli at the scale of a single whisker and on the influence on this integration of the sensory context, which can be modulated e.g. by the spatio-temporal correlation of all whiskers.

To address these questions, our group develops new experimental tools that allow in two-photon fluorescence microscopy the optimal recording of neuronal network activity in vivo on anaesthetized or behaving animals: adaptive optics to improve imaging depth, ultrafast scans using acousto-optic deflectors to increase the temporal resolution of the recordings as well as their signal to noise ratio, and optical coherent tomography to visualize myelinated axons without labeling.

Membres de l'équipe

Wang Jinyu
Paresys Gérard
Léger Jean François
Ventalon Cathie

Lab rotation

Adaptive changes in neuronal receptive fields under multiple contexts

Chercheur responsable: 

BOURDIEU Laurent

Dates: 

1 January 2017 - 31 March 2017

Date limite de candidature: 

1 January 2017

Lab rotation proposal: 3 months

~ Jan-March 2017 ~ Apr-June 2017

Project

We propose to study how cortical receptive fields can be rapidly reshaped by behavioral conditioning. We will use two-photon fluorescence microscopy to record neuronal activity with cellular resolution in anaesthetized and in awake mice. We will focus on adaptive plasticity in receptive fields of neurons in A1 (the primary auditory cortex), and explore how classical conditioning for a single tone can change the receptive field properties of both inhibitory interneurons and excitatory pyramidal cells in layers I, to III of the cortex. This is a collaborative project between our group and Dr. Shihab Shamma’s group at the DEC department (ENS). These experiments will provide a better understanding of the cellular basis of adaptive neuronal changes during classical conditioning, and also yield new insights into the effects of learning and reward on early sensory processing.

Address: IBENS - École Normale Supérieure - 46, Rue d’Ulm 75005 Paris 

Phone number: +33 1 44 32 37 34 ; Emailjean-francois.leger@ens.fr

Website

Superviseur: 

Jean-François LEGER

Multi-whisker receptive fields and sensory-motor integration

Chercheur responsable: 

BOURDIEU Laurent

Dates: 

1 January 2017 - 31 March 2017

Date limite de candidature: 

1 January 2017

Lab rotation proposal: 3 months

~ Jan-March 2017 ~ Apr-June 2017



Address: IBENS - École Normale Supérieure - 46, Rue d’Ulm 75005 Paris 

Phone number: +33 1 44 32 37 34 ; Emaillaurent.bourdieu@ens.fr

Website

 

Superviseur: 

Laurent BOURDIEU