Morphogenesis of the vertebrate brain

Research center

9 Quai Saint-Bernard Université Pierre et Marie Curie, Campus Jussieu, Bâtiments A-B-C
75005 Paris
Michel Labouesse

Institution

CNRS
Université Pierre et Marie Curie
ED158
Université Pierre et Marie Curie

Laboratory

Laboratoire de Biologie du Développement LBD
UMR 7622

Mots clefs

Morphogenèse du système nerveux, neurogénèse, cil primaire, signalisation, axone
Available to host a PhD student

publications

Burcklé C., Gaudé H-M., Vesque C., Silbermann F., Salomon R., Jeanpierre C., Antignac C., Saunier S. and Schneider-Maunoury S. 2011. « Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros ». Human Molecular Genetics 20 :2611-2627. doi: 10.1093/hmg/ddr164.

Besse L., Neti M., Anselme I., Gerhardt C., Rüther U., Laclef C., and Schneider-Maunoury S. 2011. « Primary cilia control telencephalic patterning and morphogenesis via Gli3 proteolytic processing ». Development  138:2079-88.

Stedman A, Lecaudey V, Havis E, Anselme I, Wassef M, Gilardi-Hebenstreit P, Schneider-Maunoury S. 2009. A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3. Dev Biol. 327:566-77.

 Han YG, Spassky N, Romaguera-Ros M, Garcia-Verdugo JM, Aguilar A, Schneider-Maunoury S, Alvarez-Buylla A. 2008. Hedgehog signaling and primary cilia are required for the formation of adult neural stem cells.  Nat Neurosci. 11:277-84.

DelousM, BaalaL, SalomonR, Laclef C, VierkottenJ, ToryK, GolzioC, LacosteT, BesseL, OzilouC, MoutkineI, HellmanNE, AnselmeI, SilbermannF, Vesque C,(…) Schneider-Maunoury S, Attié-Bitach T and Saunier S.2007. The novel ciliary gene FTM is mutated in cerebello-oculo-renal syndrome (Joubert syndrome type B) and Meckel syndrome. Nature Genetics, 39 p. 875-881. News and Views dans Nature Genetics, 39 p. 818-19.

Fields of research

Neurogenetics / neurodevelopment

Research Theme

Our group is interested in the molecular and cellular mechanisms underlying brain morphogenesis in vertebrates, and in understanding how these mechanisms are perturbed in human diseases.

To investigate these processes, we take advantage of two complementary model organisms, the mouse and the zebrafish. We have three main research axes.

1) We study the gene regulatory hierarchies involved in the early subdivision of the neural plate, the future central nervous system, during gastrulation.

2) We investigate the function of primary cilia in brain morphogenesis. Specifically, we study the Rpgrip1L gene, which codes for a protein localized at the ciliary transition zone and whose mutations have been identified in two human ciliopathies, Joubert and Meckel syndromes.

3) We have demonstrated the presence of axonal transport of mRNAs in zebrafish embryos and we currently study its mechanisms and functions

Membres de l'équipe

VESQUE Christine
LACLEF Christine
ANSELME Isabelle

Lab rotation

Coordination of morphogenesis with axonogenesis in the developing olfactory placode

Chercheur responsable: 

ROUYER François

Dates: 

1 September 2016 - 30 June 2017

Date limite de candidature: 

1 September 2016

Lab Rotation:

~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017

Project: 

Drosophila has about 150 clock neurons that control time-of-day information and orchestrate circadian behavioral rhythms such as sleep-wake cycles. Different periods of diurnal time, e.g., morning and evening, are encoded by distinct neuronal subpopulations. We are interested in (a) deciphering the pathway of information flow from these master clock neurons, (b) and in understanding how the downstream circuit decodes temporal information. To this end we have selected transgenic lines that express Gal4/LexA transgenes in specific populatins of neurons whose anatomical location in the brain suggests possible connections with clock neurons. These transgenes allow the considered neurons to be analyzed anatomically and functionally. The project will focus on neurons that might be downstream targets of the clock neurons that contribute to the evening peak of activity in light:dark cycles. We will use molecular-genetic tools to anatomically and functionally verify whether and how these putative downstream neurons are connected different types of evening clock neurons. The behavioral contribution of these neurons will be analyzed by manipulating their activity (hyperexcitation or silencing) in different environmental conditions. The experiments will involve Drosophila genetics, behavioral analysis (sleep-wake cycles), as well as anatomical and functional imaging (Ca2+) of the clock/target neurons.

Address: Paris-Saclay Institut of Neuroscience 1 av. de la terrasse, 91190 Gif-sur-Yvette

Phone: 33 1 69 82 34 36

Email: rouyer@inaf.cnrs-gif.fr

Website



Superviseur: 

François ROUYER