Development and Evolution of the Forebrain (DECA)

Leader

Research center

Bâtiment 145, Centre d'études de Saclay
91191 Gif sur Yvette
Philippe Vernier

Institution

CNRS
Université Paris Sud
ED 568 BioSigNe
Université Paris Sud

Laboratory

Département Développement-Évolution
UMR9197
Idex NeuroSaclay

Mots clefs

Development, Evolution, Behavior, Adaptation, Evolutionary forces
Available to host a PhD student

publications

Hinaux H, Devos L, Blin M, Elipot Y, Bibliowicz J, Alié A, Rétaux S. Sensory evolution in blind cavefish is driven by early embryonic events during gastrulation and neurulation. Development. 2016 Dec 1;143(23):4521-4532. 

Yannick Elipot, Hélène Hinaux, Jacques Callebert, Jean-Marie Launay, Maryline Blin and Sylvie Rétaux (2014) A Mutation in theEnzyme Mono Amine Oxidase explains part of the Astyanax Cavefish Behavioural Syndrome. Nature Communications, 5:3647. IF10.

Yannick Elipot, Laurent Legendre, Stéphane Père, Frédéric Sohm and Sylvie Rétaux (2014) Astyanax transgenesis and husbandry:how cavefish enters the lab. Zebrafish, 11(4):291-9. IF3 (with cover picture)

Luis Espinasa, Jonathan Bibliowicz, William R. Jeffery, and Sylvie Rétaux. (2014) Enhanced prey capture skills in Astyanax cavefishlarvae are independent from eye loss. Evo Devo 5: 35.

Suzanne E. McGaugh, Joshua B. Gross, Bronwen Aken, Maryline Blin, Richard Borowsky, Domitille Chalopin, Hélène Hinaux, WilliamJeffery, Alex Keene, Li Ma, Pat Minx, Daniel Murphy, Kelly E. O’Quin, Sylvie Rétaux, Nicolas Rohner, Steve M. J. Searle, BethanyStahl, Cliff Tabin, Jean-Nicolas Volff, Masato Yoshizawa, Wes C. Warren. (2014) The cavefish genome reveals candidate genes foreye loss. Nature Communications, Oct 20; 5:5307.

Hélène Hinaux, Maryline Blin, Julien Fumey, Laurent Legendre, Didier Casane and Sylvie Rétaux (2014) Lens defects in Astyanaxmexicanus cavefish: focus on crystallin evolution and function. Developmental Neurobiology, 28. [Epub ahead of print].

Fields of research

Neurogenetics / neurodevelopment

Research Theme

We address two questions:

1) Which mechanisms underly the evolution and the diversification of the vertebrate forebrain?

2) What are the evolutionary forces involved?

To answer these so-called "evo-devo" and evolutionary biology questions, we have chosen an original animal model, the fish Astyanax mexicanus. Within this species, there are several populations/morphotypes, including "normal" river-dwelling fish and blind cave-leaving animals, which have lost their eyes and their pigmentation in the course of their evolution and adptation to the total and permanent darkness of caves. Astyanax cavefish have undergone some losses (eyes, pigmentation) but have also evolved a number of morphological (size and form of various brain regions, number of teeth, size of jaws...) physiological (yolk size, metabolism) and behavioral (social, feeding, sleep, sensory...) changes, some which probably being adaptative.

To answer the first question, we compare embryonic and larval development in Astyanax surface fish and cavefish. We compare expression patterns for genes controling signaling, regionalisation and neural patterning (through in situ hybridisation). We compare morphogenetic movements in the two types of embryos (4D imaging). We compare neurogenic properties of progenitors(incorporations of analogs). We manipulate gene expression (transgenesis or pharmocological tools). Our current projects focus on the comparative development of two particular neural systems/circuits: the olfactory system, which is more developped in cavefish, probabaly as a sensory compensation of the loss of vision; and the hypothalamus, in which the aminergic and peptidergic neuronal types have evolved (in numbers) between the two Astyanax populations. Finally, our aim is to link the developmental evoltuion of these neural systems to the behaviors they govern. To this end, we have set up sensitive behavioral assays (agressive behavior, feeding behavior, olfactory capabilities).

To answer the second question, we perform molecular evolution analyses. We search transcriptome-wide or genome-wide for mutational patterns and polymorphisms between cavefish and surface fish. As an example, in cavefish we can observe an accumulation of mutations in genes expressed during eye and lens development. Such result suggest relaxed selection on "eye genes" and genetic drift as a mechanisms underlying the evolutionary loss of eyes in cavefish. The identified mutations also can be considered as good candidates to explain the developmental degenerescence of the eyes in cavefish.

Membres de l'équipe

Maryline Blin
Stéphane Père

Lab rotation

Morphogenesis of the anterior neural plate in blind cavefish

Chercheur responsable: 

RETAUX Sylvie

Dates: 

2 January 2018 - 29 June 2018

Date limite de candidature: 

29 June 2018

Period

~ Jan-March 2018

~ April-June 2018

Project

We use the blind cavefish morph of the species Astyanax mexicanus to address developmental mechanisms of morphological evolution. Anterior neural plate morphogenesis will be compared in cavefish (“natural mutant”) and surface fish (“wild-type”) of the same species, using transgenic reporter lines and live imaging, to understand how differences in optic vesicle formation and basal forebrain development are set up, early in embryogenesis, between the two Astyanax morphotypes.  

Contact

Neuroscience Paris-Saclay Institute - Avenue de la terrasse 91190 Gif sur Yvette - +33 1 69 82 34 52 - retaux@inaf.cnrs-gif.fr ; jorge.torres-paz@cnrs.fr 

Superviseur: 

TORRES-PAZ Jorge