Neuronal circuit dynamics & behaviour

Leader

Research center

45 rue d’Ulm
75230 Paris
Marc Mézard

Institution

ENS
CNRS
Inserm
ED158
Université Pierre et Marie Curie

Laboratory

Ecole Normale Superieure, Institut de Biologie de l'ENS IBENS
U1024 UMR 8197
Labex Memolife, Idex PSL

Mots clefs

zebrafish
neural circuits
Optogenetics
Two-photon calcium imaging
motor behaviour
cognitive functions
ongoing spontaneous activity
SPIM
light-sheet imaging
Available to host a PhD student

publications

 Pérez-Schuster V, Kulkarni A, Nouvian M, Romano SA, Lygdas K, Jouary A, Dipoppa M, Pietri T, Haudrechy M, Candat V, Boulanger-Weill J, Hakim V, Sumbre G. Sustained Rhythmic Brain Activity Underlies Visual Motion Perception in Zebrafish. Cell Reports. 17, 4:1098-1112. doi: 10.1016/j.celrep.2016.09.065.

.   Jouary A, Haudrechy M, Candelier R, Sumbre G. A 2D virtual reality system for visual goal-driven navigation in zebrafish larvae.Scientific Reports. 6, 34015; doi: 10.1038/srep34015

.   Jouary A and Sumbre G. Automatic classification of behavior in zebrafish larvae. BioRxiv. doi: http://dx.doi.org/10.1101/052324

.  Candelier R*, Murmu MS*, Romano SA, Jouary A, Debregeas G*, Sumbre G*. (2015) A microfluidic device to study neuronal and motor responses to acute chemical stimuli in zebrafish. Scientific Reports. 5, 12196; doi: 10.1038/srep12196

Romano SA, Pietri T, Perez-Schuster V, Jouary A, Haudrechy M, Sumbre G. (2015) Spontaneous Neuronal Network Dynamics Reveal Circuit’s Functional Adaptations for Behavior. Neuron. http://dx.doi.org/10.1016/j.neuron.2015.01.027

Pietri T, Roman AC, Guyon N, Romano SA, Washbourne P, Moens CB, de Polavieja GG, Sumbre G. (2013) The first mecp2-null zebrafish model shows altered motor behaviors. Front. Neural Circuits.7(118) doi:10.3389/fncir.2013.00118

Panier T, Romano S, Olive R, Pietri T, Sumbre G, Candelier R and Debrégeas. (2013) Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy. Front. Neural Circuits. 7(65) doi: 10.3389/fncir.2013.00065

Lim BK, Cho SJ, Sumbre G, Poo MM. (2010) Region-specific contribution of ephrin-B and Wnt signaling to receptive field plasticity in developing optic tectum. Neuron. 65(6):899-911.

Zullo L, Sumbre G, Angisola C, Flash T and Hochner B. (2009) Non-somatotopic organization of the higher motor centers in octopus. Current Biology. 19(19):1632-1636.

 Sumbre G, Muto A, Baier H and Poo MM. (2008) Entrained rhythmic activities of neuronal ensembles as perceptual memory of time interval. Nature 456(7218):102-106 (AOP).

Fields of research

Neurophysiology / systems neuroscience

Research Theme

Using the zebrafish larva as the experimental model and a multidisciplinary approach, including twophoton calcium imaging to monitor activity of neural networks, motor behaviours, genetic engineering techniques to label, monitor and manipulate activity of specific neurons or entire circuits and mathematical methods for data analysis, we are studying the following subjects:

1) Multimodal sensory perception:

we are studying the neural basis of sensory perception using different sensory modalities asking the question if the neural correlates of sensory perception are unique of each sensory modality or a comprehensive common one for all modalities. More precisely, we are using visual illusion to study the neural network mechanisms that enable the emergence of visual perception. In parallel, we are using microfluidic devices to present gustatory stimuli with unprecedented spatiotemporal resolution, permitting to present rapid, non-perceived, stimuli and longer, perceived stimuli.

2) Ongoing spontaneous activities:

In absence of sensory stimulation sensory brain areas remain active. These ongoing spontaneous (OS) neuronal activities were traditionally considered as random independent biophysical noise with no functional value for brain computations. However, in the last decades it was shown that OS activities are capable of interacting meaningfully with sensory-induced neuronal inputs, both at the single neuron and the network level. OS activities are structured according to the coarse functional and anatomical circuitry and partially account for both the variability of stimulus-evoked neuronal responses and fluctuations in human motor behaviour . Furthermore, it was suggested that neuronal responses to stimuli are only mild modulations of OS activity structure, the latter representing an internal statistical model of the environment that samples the constrained repertoire of possible neuronal responses. Nevertheless, the origin of this structured OS activities and its biological relevance still remain elusive. Using zebrafish larvae we showed for the first time, the fine structure of the coherent OS activities of a significant fraction of the largest sensory brain region in an intact, awake, vertebrate. More specifically, we have found that the OS network activities showed spatiotemporal dynamics organised in distinct neuronal assemblies. These OS assemblies, primarily composed of neighbouring neurons, reconstructed the OT functional retinotopic map. These neuronal assemblies were tuned to visual objects corresponding to the angular size of zebrafish's natural prey at different positions in the larva's field of view (FoV), and showed angular tuning preferences that resembled prey-detection performance. Moreover, these assemblies consisted of ?preferred? network states, generated through a winner-take-all mechanism and attractor-like dynamics. Therefore, the OT coherent OS activities emerge from its intrinsic circuitry, optimised for a vital behaviour (prey detection),

3) Functional incorporation of new-born neurons to already established neural circuits:

Newborn neurons originate from a process called neurogenesis. This process is defined by the division of neural stem cells (NSCs) into daughter cells that then differentiate, migrate and give rise to functional neurons. Several observations highlight the requirement of electrical activity of the migrating neurons to sustain their survival and integration). However, the neurophysiological mechanisms underlying their incorporation into functional neural circuits remain elusive. For this purpose, we are using state-of-the-art genetic techniques to label new-born neurons while we monitor their morphology and activity, as well as the activity of the neighbouring matured neurons, while presenting visual stimuli or recording spontaneous activities. So far, we can follow the functional connectivity map during development, where some random connections appear during the early stages, while later, a connectivity pattern becomes evident, forming functional assemblies as those observed to emerge from the dynamics of the ongoing spontaneous activities (project 1).

Lab rotation

Optogenetics to study principle of functional connectivity among neurons in large circuits

Chercheur responsable: 

SUMBRE German

Dates: 

18 September 2017 - 29 June 2018

Date limite de candidature: 

29 June 2018

Period

~ Sept-Dec 2017

~ Jan-March 2018

~ April-June 2018

Project

Attractor neuronal circuits are recurrently connected networks whose temporal dynamics converge and settle to  stable patterns. Theoretical attractor models have been used to explain cognitive functions and motor behaviour.

However, limited experimental evidence supports their existence, and the biological properties of the attractor circuits, are still unknown.

We have recently shown that the optic tectum of the zebrafish larva is functionally organized according to neuronal assemblies (groups of  highly correlated neurons). These assemblies showed: 1) all-or-nothing synergistic facilitation. 2) inter-assemblies competitive reciprocal inhibition generating single “winners”.  

Here, we propose to study the spatiotemporal dynamics and functional connectivity of the tectal neuronal assemblie. For this purpose, we will use two-photon calcium imaging of GCaMP6 transgenic larvae to monitor the neuronal dynamics in large neuronal circuits, while stimulating single identified neurons using optogenetics. These experiments will enable us to study the functional role of different neurons in the dynamics of the attractor circuits and learn about their connectivity patterns.

Contact

Ecole Normale Supérieure - IBENS - 46, rue d’Ulm 75005 Paris - +33 1 44 32 23 67 - sumbre@biologie.ens.fr

Superviseur: 

SUMBRE German