Mechanisms of altered rhythmogenesis in the epileptic hippocampal cortex
Dates:1 September 2016 - 30 June 2017
Date limite de candidature:1 September 2016
Lab rotation proposal: 3 months
~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017
We explore the cellular mechanisms leading to altered network activity in the epileptic brain. In particular, we focus on the role of a chloride transporter (KCC2), the expression of which is suppressed in epilepsy as well as many other related disorders. Our goal is to test whether chloride transport may represent a promising therapeutic target in the treatment of some forms of pharmaco-resistant epilepsy such as temporal lobe epilepsy. We use a viral-based approach to suppress KCC2 expression in vivo in the rat hippocampus and study how this may affect network activity. During the rotation, the student will be initiated to multisite, extracellular recordings from hippocampal slices in vitro. We will test how KCC2 extinction influences normal rhythmogenesis in the hippocampal cortex, with a specific focus on sharp-wave ripple activity. We will also test whether anomalous, rhythmic activities may be induced by KCC2 extinction and may represent the substrate for epileptiform activities in vivo. At least basic notions in electrophysiology are required for this rotation.
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