Physiology of NG2 cells

Research center

45 rue des Saints Pères
75006 Paris
Daniel Zytnicki

Institution

Inserm
Université Paris Descartes
ED157 GC2ID
Université Paris Descartes

Laboratory

Neurophysiology & New Microscopies Laboratory
Phone: (+33) 1 70 64 99 35
U1128

Mots clefs

Patch clamp
Optogenetics
Available to host a PhD student

publications

Balia M, Vélez-Fort M, Passlick S, Schäfer C, Audinat E, Steinhäuser C, Seifert G, Angulo MC (2013) Postnatal Down-Regulation of the GABAA Receptor γ2 Subunit in Neocortical NG2 Cells Accompanies Synaptic-to-Extrasynaptic Switch in the GABAergic Transmission Mode. Cereb Cortex. doi: 10.1093/cercor/bht309

 Maldonado PP, Vélez-Fort M, Levavasseur F, Angulo MC (2013) Oligodendrocyte precursor cells are accurate sensors of local K+in mature gray matter. Journal of Neuroscience 33(6):2432-42

Vélez-Fort, M, Audinat, E, Angulo, MC (2012) The central role of GABA in neuron?glia interactions. The Neuroscientist, 18(3):237-50 (Invited review ; Cover)

Vélez-Fort M, Maldonado PP, Butt AM, Audinat E, Angulo MC (2010) Postnatal switch from synaptic to extrasynaptic transmission between interneurons and NG2 cells. Journal of Neuroscience 30(20):6921-9

Zahid M*, Vélez-Fort M*, Papagiakoumou E, Ventalon C, Angulo MC, Emiliani, V (2010) Holographic photolysis to stimulate multiple cells in brain slices. PLoS One 5(2) :e9431 *Co-first authors

Vélez-Fort M, Audinat E, Angulo MC (2009) Functional ?7-containing nicotinic receptors of NG2-expressing cells in the hippocampus. Glia, 57:1104-14

Fields of research

Neurophysiology / systems neuroscience

Research Theme

Oligodendrocyte precursor cells expressing the chondroitin sulfate proteoglycan NG2, also called NG2 cells, have the ability to proliferate in the postnatal brain to generate oligodendrocytes in grey and white matters. NG2 cells play a critical role in myelination during postnatal brain development, but a pool of these progenitors is maintained in the adult and recruited to lesions in demyelinating diseases. Recent discoveries have demonstrated that NG2 cells are contacted by functional glutamatergic and GABAergic synapses from neurons in grey and white matters. The function of these synapses is still elusive. Our group is interested in the functional properties and role of neuron-NG2 cell synapses as well as in exploring new neuronal signaling mechanisms controlling NG2 cell activity in the normal and injured brains. Our research program consists of studying these synapses in the somatosensory cortex (grey matter) and corpus callosum (white matter) to dissect the synaptic connectivity of NG2 cells and decipher whether neuronal synaptic inputs control NG2 cell fate in an activity-dependent manner. We use a multidisciplinary approach that combines patch-clamp recordings, calcium imaging, immunostaining techniques, optogenetics and advanced optical methods to analyze NG2 cell physiology in acute slices and in vivo during postnatal development and a demyelination/remyelination process. Our studies may bring new perspectives on the roles played by NG2 cells in the brain and for the design of innovative therapies promoting myelin repair in demyelinating diseases.

Lab rotation

Regulation of myelin repair by glutamatergic transmission using an electropysiological and optogenetic approach

Chercheur responsable: 

ANGULO Maria Cecilia

Dates: 

1 September 2016 - 1 December 2016

Date limite de candidature: 

1 September 2016

Lab rotation proposal: 3 months 

~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017 

AddressNeurophysiology & New Microscopies Laboratory - 45, rue des Saints Pères 75006 Paris

Phone number+33 1 70 64 99 35 ; Emailmaria-cecilia.angulo@parisdescartes.fr

Website

Oligodendrocyte precursor cells (or NG2 cells) constitute a major source of remyelinating oligodendrocytes in demyelinating diseases. Recent advances have shown that these non-neuronal cells are contacted by through glutamatergic synapses from neurons, but the role of these unique neuron-glia synapses of the brain is unknown. It has been suggested that glutamatergic synaptic transmission onto NG2 cells might regulate proliferation and differentiation of these cells, and thus possibly impact myelination and myelin repair (two fundamental processes needed to improve action potential conduction). In this project, we will evaluate the effect of neuronal activity on myelin repair in a mouse model of demyelination. We will combine patch-clamp recordings with a novel optogenetic approach allowing for targeted stimulation of axons with light.

Superviseur: 

Maria Cecilia ANGULO