Imagerie dynamique du neurone

Research center

25 rue du Docteur Roux
75015 Paris
Christian Bréchot

Institution

CNRS
ED3C
Université Pierre et Marie Curie

Laboratory

Genes, Synapses, Cognition
Phone: 01 42 86 41 61
UMR 3571

Mots clefs

Cerebellum
Synapses
Dendrites
Superresolution Microscospy
Optophysiology
Available to host a PhD student

publications

DiGregorio D. Confocal spot detection of presynaptic Ca²⁺ domains. PLoS Biol. 2014 Jul 8;12(7):e1001903. doi: 10.1371/journal.pbio.1001903. eCollection 2014 Jul.

Tran-Van-Minh A, Abrahamsson T, Cathala L, DiGregorio DA. Differential Dendritic Integration of Synaptic Potentials and Calcium in Cerebellar Interneurons. Neuron. 2016 Aug 17;91(4):837-50. doi: 10.1016/j.neuron.2016.07.029.

Fink AE., Bender, KJ., Trussell, LO., Otis, TS. and DiGregorio, DA. Two-photon compatibility and single-voxel, single-trial detection of subthreshold neuronal activity by a two-component optical voltage sensor. PLoS One, in press (2012).

Abrahamsson, T.,  Cathala,  L., Matsui, K., Shigemoto, R., and DiGregorio, DA. Thin dendrites of cerebellar interneurons confer sublinear synaptic integration and a gradient of short-term plasticity. Neuron, 73: 1159-1172 (2012).

DiGregorio DA. 2011. Confocal spot detection of presynaptic Ca2+ domains. In: Imaging in neuroscience: A laboratory manual (ed. Helmchen F, Konnerth A, Yuste R), pp. 141-150. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Bradley, J., Luo, R., Otis, T.S. and DiGregorio, D.A., Submillisecond optical reporting of membrane potential in situ using a neuronal tracer dye. Journal of Neuroscience, 29(29):9197–9209 (2009).

Lutz, C, Otis, T, DeSars, V., Charpak S., DiGregorio, D. A., and Emiliani, V. Holographic photolysis of caged neurotransmitters. Nature Methods, 5(9):821-827 (2008).

Fields of research

Neuropharmacology / cell signaling

Research Theme

 Understanding how information is processed within neuronal networks requires knowledge of how neurons use cellular and molecular mechanisms to transform information communicated by synapses. Defects in the communication between synapses are thought to be at the heart of the memory deficits associated with neuropathological disorders such as Alzheimer’s disease and autism. Therefore, elucidating the properties of synapses and their ability to adapt the strength of such communication is fundamental to our understanding of brain function, learning, and memory storage under normal and pathological conditions. 

 Using advanced optical techniques to monitor and manipulate synaptic signaling in thin dendrites, somata and presynaptic boutons, we hope to identify new cellular mechanisms that define computational rules within cerebellar microcircuits. The crystalline cytoarchitecture of the cerebellum makes it ideal for studying the mechanisms influencing microcircuit computations. Because the cerebellum integrates various sensory inputs in order to fine-tune motor movements, the mechanisms and rules of multi-sensory computations identified from proposed experiments are likely to be relevant for microcircuit function throughout the brain.

 Although the cytoarchitecture and synaptic connections within the cerebellar cortex are well known, surprisingly little is known about how this microcircuit uses its molecular, cellular, and anatomical properties to dynamically process sensory information. Our previous publications and preliminary data (see below) have identified novel cellular mechanisms influencing information flow within the cerebellum, and form the basis of experiments proposed here. Using a multi-disciplinary team of physicists and biologists (as well collaborators specializing in computational neuroscience), we will combine state-of-the-art optical techniques (conventional confocal and 2-photon imaging), holographic photolysis, smart scanning (x, y and z dimensions), and superresolution imaging to study the cellular and molecular underpinnings of microcircuit computations.

Membres de l'équipe

REBOLA Nelson
MONERON Gael
SAINT-CLOMENT Cecile
TRAN-VAN-MINH Alexandra
NAKAMURA Yukihiro
REPAK Emilienne
BRANDT Maryse
CHABROL François