Dynamique et Physiopathologie des Réseaux Neuronaux

Leader

Research center

11 place Marcelin Berthelot
75231 Paris
Serge Haroche

Institution

Collège de France
CNRS
Inserm
ED158
Université Pierre et Marie Curie

Laboratory

Centre Interdisciplinaire Recherche Biologie
Phone: 01 44 27 12 26
UMR 7241 - U1050
LabEx MemoLife

Mots clefs

Synaptic plasticity
dopamine
Learning and Memory
Parkinson’s disease
neuronal networks
basal ganglia
GABAergic interneurons
endocannabinoids
Available to host a PhD student

publications

Paille V#, Fino E#, Du K, Morera-Herreras T##, Perez S#, Hellgren Kotaleski J & Venance L# (2013) GABAergic circuits control spike-timing-dependent plasticity. J Neurosci. 33:9353-9363.

Nelson MJ, Bosch C#, Venance L# & Pouget P (2013) Microscale inhomogeneity of brain tissue distorts electrical signal propagation. J Neurosci. 33(7):2821-7.

Bosch C#, Mailly P, Degos B#, Deniau JM# & Venance L #(2012) Preservation of the hyperdirect pathway of basal ganglia in a rodent brain slice. Neuroscience 215, 31-41.

Evans RC, Morera-Herreras T#, Cui Y#, Du K, Sheehan T, Kotaleski JH, Venance L# & Blackwell KT (2012) The effects of NMDA subunit composition on calcium influx and spike timing-dependent plasticity in striatal medium spiny neurons. PLoS Comput Biol. 8, e1002493.

Puente N, Cui Y#, Lassalle O, Lafourcade M, Georges F, Venance L#*, Grandes P* & Manzoni OJ* (2011) Polymodal activation of the endocannabinoid system in the extended amygdala. Nature Neuroscience 14(12), 1542-7. *: co-senior authors Pandolfo, P, Silveirinha, V, dos Santos-Rodrigues, A, Venance, L#, Ledent, C, Takahashi, RN, Cunha, RA & Köfalvi, A 2011

Cannabinoids inhibit the synaptic uptake of adenosine and dopamine in the rat and mouse striatum?, Eur J Pharmacol. Vol.655(1-3), pp. 38-45.

Fields of research

Neurophysiology / systems neuroscience

Research Theme

Nos projets de recherche concernent le codage de la mémoire et l’apprentissage dans les ganglions de la base, un ensemble de noyaux sous-corticaux impliqués dans le contrôle adaptif du comportement. Les ganglions de la base, connectés en retour au cortex cérébral et au système limbique, participent a la sélection d’actions en relation avec la motivation et la prédiction de récompense.

La pathophysiologie des ganglions de la base conduit à des désordres moteurs et cognitifs majeurs (maladie de Parkinson, TOCs, syndrome de Tourette, addiction, …) pour lesquels il n’existe pas de traitements satisfaisants jusqu’à présent.
Nous étudions différents aspects des interactions synaptiques sous-tendant les propriétés dynamiques du réseau des ganglions de la base en conditions physiologiques et pathologiques. Nous utilisons une approche multidisciplinaire combinant l’électrophysiologie (multi-patch-clamp in vitro et enregistrements in vivo), la fast-cyclic voltammétrie, l’imagerie bi-photonique, la single-cell RT-PCR et l’immunohistochimie. La variété des expertises dans notre équipe ainsi que les collaborations que nous avons établies avec des mathématiciens, des biologistes moléculaires, des biochimistes et des cliniciens, nous permet d’analyser les fonctions physiologiques et pathologiques des ganglions de la base à différents niveaux de complexité du réseau neuronal. Les principaux projets sont:
1) Les propriétés dynamiques et la plasticité synaptique (STDP) dans les réseaux neuronaux des ganglions de la base et du cortex.

2) Les interactions neurone-glie : nuos analysons la contribution de la recapture de neurotransmetteurs par les astrocytes dans le traitement des informations cortico-striatales.

3) Les propriétés neuromodulatrices de la dopamine et des endocannabinoides.

Lab rotation

Dopaminergic modulation of striatal electrical synapses

Chercheur responsable: 

VENANCE Laurent

Dates: 

1 September 2016 - 30 June 2017

Date limite de candidature: 

1 September 2016

Lab Rotation:

~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017

Project: 

In Parkinson’s disease, caused by a deficit of dopamine, deleterious synchronous activities (beta-band) are recorded throughout basal ganglia. Synchronous activities can arise from electrically-coupled GABAergic interneuron networks. Gap junctions between neurons underlie electrical synapses. The project consists in recording electrical synapses between striatal GABAergic interneurons with double patch-clamp and evaluating their modulation by dopamine in physiological conditions and a rodent model of Parkinson’s disease. The state of electrical coupling will be put in relation with synchronous activities to determine the role of neuronal gap junctions onto deleterious synchronous activities (beta-band) in Parkinson’s disease. Techniques: electrophysiology (double patch-clamp in vitro), optognetics, two-photon calcium imaging, multi-channel recordings in vivo, Parkinson’s disease rodent model, computational modeling (in coll).

AddressCollège de France - 11, Place Marcelin Berthelot 75231 Paris Cedex 05

Phone number: +33 1 44 27 12 26 ; Emaillaurent.venance@college-de-france.fr

Website

Superviseur: 

Laurent VENANCE

Dopaminergic control of cortical and thalamic interplay for striatal synaptic plasticity

Chercheur responsable: 

VENANCE Laurent

Dates: 

1 September 2016 - 30 June 2017

Date limite de candidature: 

1 September 2016

Lab rotation proposal

~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017

Project:

Basal ganglia are involved procedural learning and memory. Striatum, the main inputs nucleus of basal ganglia, acts as a coincidence detector of cortical and thalamic inputs: striatum extracts pertinent information from background noise. Dopamine tunes this striatal detection threshold. We have pioneered the field of spike-timing dependent plasticity (STDP), a synaptic Hebbian learning rule, at the level of basal ganglia (Fino et al., 2005; Fino et al., 2010; Paillé et al., 2013; Cui et al., 2016). The project is to explore the impact on striatal synaptic plasticity of cortical and thalamic activities timed with dopamine opto-stimulation. This will be achieved in vitro and in vivo in physiological conditions and in a rodent model of Parkinson’s disease. This project aims at a better understanding how cortical and thalamic activity together with dopamine concurred to striatal synaptic plasticity and the engram of procedural learning.Techniques: electrophysiology (in slices and in vivo patch-clamp), 2-photon microscopy, optognetics, multi-channel recordings.

AddressCollège de France - 11, Place Marcelin Berthelot 75231 Paris Cedex 05

Phone number: +33 1 44 27 12 26 ; Emaillaurent.venance@college-de-france.fr

Website

Superviseur: 

Laurent VENANCE