Oligodendroglial, microglial interactions during demyelination and remyelination in a Xenopus transgenic model
Dates:2 January 2018 - 30 March 2018
Date limite de candidature:30 March 2018
~ Jan-March 2018
We have generated a transgenic Xenopus of conditional demyelination in which myelinating oligodendrocytes express the GFP reporter. We are now using the Crispr/Cas9 knock-in approach to introduce the tdTomato fluorescent reporter into the microglial lineage of this transgenic Xenopus line. Fluorescent microglial cells (red) and oligodendrocytes (green) will allow us to monitor in vivo, thanks to the transparency of tadpoles, microglial activation following demyelination and remyelination.
Institut du Cerveau et de la Moelle épinière - 47, boulevard de l'Hôpital 75651 Paris cedex 13 - +33 1 57 27 44 71 - firstname.lastname@example.org