Identify how early neuronal activity regulates neural stem cell fate
Dates:1 September 2016 - 30 June 2017
Date limite de candidature:1 September 2016
~ Sep-Dec 2016 ~ Jan-March 2017 ~ Apr-June 2017
Oligodendrocytes play a central role in neural physiology by forming myelin sheaths around axons. They arise as oligodendrocyte precursor cells (OPCs) from neural stem cells (NSCs) in the embryo but also after a demyelinating injury at adulthood where they contribute to regenerate myelin. The project aims at unraveling the role of spontaneous neuronal activity and activity-dependent signaling molecules in controlling commitment of NSCs to the OPC lineage. We will use the embryonic chicken spinal cord as a simple and reliable model to manipulate neuronal activity in vivo and analyze the consequences of these perturbations on OPC commitment. Neurotransmitter receptors and voltage-gated channels expressed by NSCs will be characterized by patch-clamp recordings and their specific influence on the glial fate choice of NSCs will be evaluated. Such knowledge should have clinical output in demyelinating pathologies but also in apprehending, and ultimately preventing, the potential harmful effect of neuroactive drugs during pregnancy.
Address: Neuroscience Paris Seine - Université Pierre et Marie Curie - 9 quai Saint Bernard 75005 Paris
Phone number: +33 1 44 27 80 92 ; Email: Jeanfirstname.lastname@example.org