De la stimulation sensorielle à l'hyperémie fonctionnelle


Research center

17 rue Moreau
75012 Paris
José-Alain Sahel


Université Paris Descartes
ED 157 GC2ID / ED158
Université Paris Descartes / Université Pierre et Marie Curie


Neurophysiology & New Microscopies Laboratory
Phone: (+33) 1 42 86 41 48
Idex Sorbonne Paris Cité

Mots clefs

Microscopy of the living brain


Lyons DG, Parpaleix A, Roche M, Charpak S.Mapping oxygen concentration in the awake mouse brain.Elife. 2016 Feb 2;5. pii: e12024. doi: 10.7554/eLife.12024.

Ducros M, Houssen YG, Bradley J, de S, V, Charpak S (2013) Encoded multisite two-photon microscopy. Proc Natl Acad Sci U S A.110 13138-13143.

Parpaleix A, Houssen YG, Charpak S. (2013) Imaging local neuronal activity by monitoring Po2 transients in capillaries. Nature med,19:241-246.

Charpak S, Stefanovic B (2012) Shedding light on the BOLD fMRI response. Nat Methods 9:547-549. (News and Views)

Lecoq, J, Parpaleix, A, Roussakis, E, Ducros, M., Goulam, Y, Vinogradov, S, Charpak, S (2011) Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels. Nature Med. 17:893-898.

Najac M., De Saintjan D,Reguero L,Grandes P,Charpak S (2011) Mono- and polysynaptic feed-forward inputs to mitral cells from olfactory sensory neurons, J Neurosci. 31:8722-8729.

Fields of research

Neurophysiology / systems neuroscience

Research Theme

Our goal is to understand how a sensory stimulus such as an odor generates a population activity in a small cellular network, the olfactory bulb glomerulus. We investigate this question by using or developing optical tools that can be combined with electrophysiological recordings and allow the observation of neuron and astrocyte activities in vitro and in vivo. During the last 5 years, our research aimed in three directions:

1. The improvement of optical methods to image cellular activity, blood flow and brain metabolism

2. The study of synaptic interactions within the glomerular network

The analysis of neurovascular coupling, functional hyperaemia and energy consumption in glomeruli.